Journal: Annals of Medicine and Surgery

Article Title: Combination of cycling hyperthermia and echinacoside creates a synergistic curing effect on pancreatic cancer PANC-1 cells

doi: 10.1097/MS9.0000000000003545

Figure Lengend Snippet: Effect of Ech alone or in combination with TC-HT on the cell viability. (A) MTT viability assay of PANC-1 cells treated with different concentrations of Ech or in combination with TC-HT treatment. (B) MTT viability assay of H6c7 normal human pancreatic cells treated with 20 μM Ech or in combination with the same TC-HT treatment. Data represent the mean ± standard deviation ( n = 3). Statistical significance was determined by one-way ANOVA followed by Tukey’s post hoc test (*** P < 0.001).

Article Snippet: H6c7 human pancreatic duct epithelial cell line was obtained from Kerafast, Inc. (Absolute Biotech, Boston, MA, USA) and maintained in keratinocyte serum-free medium (Invitrogen; Thermo Fisher Scientific, Inc., Carlsbad, CA, USA) supplemented with human recombinant epidermal growth factor, bovine pituitary extract (Invitrogen; Thermo Fisher Scientific, Inc.), and 1% (v/v) penicillin and streptomycin.

Techniques: MTT Viability Assay, Standard Deviation

Journal: Cancer Research Communications

Article Title: Collagen XVII Promotes Pancreatic Ductal Adenocarcinoma Tumor Growth through Regulation of PIK3R5

doi: 10.1158/2767-9764.CRC-24-0392

Figure Lengend Snippet: Collagen XVII expression is upregulated in PDAC cells upon interaction with CAFs. A, Western blot analysis of collagen XVII expression in immortalized human pancreatic ductal cells [human pancreatic duct epithelial (HPDE)], six PDAC cell lines, and telomerase reverse transcriptase–immortalized CAFs (CAF-1). β-Actin is shown as a loading control. B, COL17A1 expression in cell lines and matching xenograft tumors. C, Representative IHC staining of collagen XVII in cell line xenografts and PDXs of MGH1319. Scale bars, 50 μm. D, Schematic of the experimental setup of coculture and FACS of PDAC and CAF-1 cells. E, Collagen XVII expression in MGH1319 cells in monoculture and after coculture with CAF-1. β-Actin is shown as a loading control. F–H, Relative COL17A1 expression in MGH1319, MGH1275, MGH1108, and CAF-1 after mono- and coculture. The average (±SEM) from three independent experiments is shown. *, P < 0.05; ****, P < 0.0001; ns, not signficant (unpaired t test).

Article Snippet: Immortalized epithelial cells from normal human pancreatic duct epithelial cells were purchased from ATCC.

Techniques: Expressing, Western Blot, Reverse Transcription, Control, Immunohistochemistry

Journal: Discover oncology

Article Title: Cancer-associated fibroblast-derived COL17A1 promotes gemcitabine resistance and tumorigenesis in pancreatic cancer cells by interacting with ACTN4.

doi: 10.1007/s12672-025-01825-8

Figure Lengend Snippet: Fig. 2 CAFs increases COL17A1 expression in GEM-resistant PC cells. A GSE192907 dataset showed that COL17A1 was highly expressed in resistant CAFs. B Western blotting analysis for COL17A1 expression in PANC-1/GR and BxPC-3/GR cells incubated with NF-CM or CAF-CM. C–E TCGA, CPTAC and GEPIA databases showed that COL17A1 expression was higher in PAAD tissues compared with the normal samples. F Kaplan–Meier Plotter predicted that high COL17A1 expression was associated with shorter survival times in PAAD patients. G qRT-PCR anal- ysis for COL17A1 expression in GEM-resistant or -sensitive PC tissues. H Western blotting analysis for COL17A1 expression in normal HPDE, parental PANC-1 and BxPC-3 cells as well as PANC-1/GR and BxPC-3/GR cells. *P < 0.05

Article Snippet: :(0123456789) Human pancreatic duct epithelial (HPDE) cell line (Cat#CL-0921, Procell) was cultured in keratinocyte serum-free medium containing epidermal growth factor, bovine pituitary extract (Cat#:17005042, Invitrogen, Carlsbad, CA, USA) and 1% penicillin/streptomycin (Cat#PB180120, Procell) at 37 °C with 5% CO2.

Techniques: Expressing, Western Blot, Incubation, Quantitative RT-PCR